Deletion and Substitution Analysis of the Escherichia coli TonB Q160 Region
نویسندگان
چکیده
منابع مشابه
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Two sets of deletions produced in vitro by S1 nuclease were used to study the structure and function of promoters lacPUV5 and lacP115. The upstream boundary of the RNA polymerase binding site in lacPUV5 was determined by comparing the levels of beta-galactoside expression in vivo programmed from a set of deletions progressively extending into the -35 region of the lacPUV5 promoter. Sequences up...
متن کاملIdentification of the Escherichia coli tonB gene product in minicells containing tonB hybrid plasmids.
Sotlillm clodtq~l sul~~t~e/polyacrylarnide gel :tnwlysis ot’ protrins t~codcd by ii sc>rics ot’ to?/ R + plasmids in minicells has icknt~ified the torLB gene product ws a prokin wit)h km apparent, molecular weight of’ 36,000. A parallel analysis of s(‘vcn tort N mnt,ations which have been genetically crosst~i onto a tonB+ plasmid support,s this itkntification; t)he 36,000 M, protein is tlbsent ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2007
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00180-07